Mutation E48K in PB1 Polymerase Subunit Improves Stability of a Candidate Live Attenuated Influenza B Virus Vaccine

Influenza B virus NS1-truncated mutants: live-attenuated vaccine approach.

Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccination remains by far the best means of protection against infections with these viruses. Here, we report the construction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates. Employing reverse genetics, we altered the NS1 gene, which encodes a type I interfer...

A promoter mutation in the haemagglutinin segment of influenza A virus generates an effective candidate live attenuated vaccine

BACKGROUND Annual seasonal and pandemic influenza vaccines need to be produced in a very tight time frame. Haemagglutinin (HA) is the major immunogenic component of influenza vaccines, and there is a lot of interest in improving candidate vaccine viruses. OBJECTIVES It has been shown elsewhere that mutations introduced in the non-coding region of influenza genome segments can upregulate prote...

Development of an Attenuated Live Virus Vaccine against Sheep Pox

λ-Red-Recombineering Live Attenuated ΔipaD Shigella dysenteriae from Iranian Isolates as A Candidate of Vaccine

Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...

Elastase-Dependant Live Attenuated Swine Influenza Virus Vaccine in Pigs

Swine influenza (SI) is an acute, highly contagious, respiratory disease of swine caused by influenza A viruses. In addition, SI infections possess significant human public health concerns as they may serve as intermediate host for the generation of new pandemic viruses. Vaccination is still the primary method for the prevention and control of SI. Currently, commercially available vaccines agai...